Hay Ison criteria for diagnosis of bacterial vaginosis

Grade 0: No bacteria present

Grade 1: Lactobacillus morphotypes predominate

Grade 2: Mixed flora with some lactobacilli present, but gardnerella or mobiluncus morphotypes also present

Grade 3*: Predominantly gardnerella and/or mobiluncus morphotypes. Few or absent lactobacilli.

Grade 4: Gram-positive cocci predominate

* Grade 3 is diagnostic of bacterial vaginosis.

Source: Ison CA, Hay PE. Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics. Sex Transm Infect 2002; 78:413.

Graphic 86620 Version 2.0

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Hay Ison criteria for diagnosis of bacterial vaginosis