Food additive | Preparation of epicutaneous skin test solution | Reference |
Gelatin | Dissolve 1 teaspoon (5 grams) of gelatin powder (plain or flavored) in 5 mL of normal saline. | Vaccine allergy practice parameter. Kelso JM, Li JT. Practice Parameters: Adverse reactions to vaccines. Ann Allergy Asthma Immunol 2009; 103:1. |
Carmine | Make 10% solution of carmine powder in phosphate-buffered saline. Agitate at 4°C for two hours. Dialyze against purified water, and filter through 0.22-micron filter. | Anibarro B, Seoane J, Vila C, et al. Occupational asthma induced by inhaled carmine among butchers. Int J Occup Med Environ Health 2003; 16:133. |
Annato | Additive-containing food (Gouda cheese) and a commercial preparation of annato dye, diluted 1000-fold was used. | Ebo DG, Ingelbrecht S, Bridts CH, et al. Allergy for cheese; evidence for an IgE-mediated reaction from the natural dye annatto. Allergy 2009; 64:1558. |
Guar gum | Additive-containing food (a meal substitute) and guar gum preparation (no details provided). | Papanikolaou I, Stenger R, Bessot JC, et al. Anaphylactic shock to guar gum (food additive E412) contained in a meal substitute. Allergy 2007; 62:822. |
Psyllium | Extract commercially available. | |
Carrageenan | 0.4% weight/volume sodium carrageenan. | Tarlo SM, Dolovich J, Listgarten C. Anaphylaxis to carrageenan: a pseudo-latex allergy. J Allergy Clin Immunol 1995 (5 part 1):933. |
Potassium metabisulfite | Maximum concentration of 1 mg/mL. | Bush RK, Zoratti E, Taylor SL. Diagnosis of sulfite and aspirin sensitivity. Clin Rev Allergy 1990; 8:159. |
Yeast (saccharomyces cerevisiae) | Extract commercially available. | |
Erythritol | 2 mg/mL, 20 mg/mL, and 200 mg/mL in distilled water. | Shirao K, Inoue M, Tokuda R, et al. "Bitter Sweet": A child case of erythritol-induced anaphylaxis. Allergol Int 2013; 62:269. |
Carob bean gum | Carob gum at 5 mg/mL and 25 mg/mL. Can use raw carob gum, boiled carob gum, and carob bean extract for skin testing also. | Alarcon, E, del Pozo MD, Bartolome B, et al. J Investig Allergol Clin Immunol 2011; 21:77. |
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