Food additive | Preparation of epicutaneous skin test solution |
Gelatin | Dissolve 1 teaspoon (5 grams) of gelatin powder (plain or flavored) in 5 mL of normal saline.[1] |
Carmine | Make 10% solution of carmine powder in phosphate-buffered saline. Agitate at 4°C for 2 hours. Dialyze against purified water, and filter through 0.22 micron filter.[2] |
Annatto | Additive-containing food (Gouda cheese) and a commercial preparation of annatto dye, diluted 1000-fold was used.[3] |
Guar gum | Additive-containing food (a meal substitute) and guar gum preparation (no details provided).[4] |
Psyllium | Extract commercially available. |
Carrageenan | 0.4% weight/volume sodium carrageenan.[5] |
Potassium metabisulfite | Maximum concentration of 1 mg/mL.[6] |
Yeast (Saccharomyces cerevisiae) | Extract commercially available. |
Erythritol | 2, 20, and 200 mg/mL in distilled water.[7] |
Carob bean gum | Carob gum at 5 and 25 mg/mL. Can use raw carob gum, boiled carob gum, and carob bean extract for skin testing also.[8] |