DAPI stain of a transverse appendix section taken at a magnification of x100. No leukocytes can be seen within the appendix confirming that the appendix is not inflamed. Bacteria are homogeneously distributed in the lumen of the appendix and clearly demarcated from the mucosa by a mucus layer.
Courtesy of Alexander Swidsinski, MD, and Vera Loening-Baucke, MD.