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Markers commonly used for assessment of lymphocyte subsets by flow cytometry

Markers commonly used for assessment of lymphocyte subsets by flow cytometry
Marker name Cell type Comment
CD3 T cells Expressed on all T cells and no other cell type
CD4 T cell subset Predominantly helper/inducer T cells
CD8 T cell subset Predominantly cytotoxic T cells; expressed by up to one-third of NK cells
CD19 or CD20 B cells  
CD16 NK cells Some NK cells may not express CD16
CD56 NK cells Expressed on the majority of NK cells
CD57 NK cells Expressed on the majority of NK cells; combinations of CD16, CD56, and CD57 will more reliably evaluate NK cell number than any marker alone
CD45RA Naïve T cells  
CD45RO Memory T cells  
CD: cluster of differentiation; NK: natural killer.
Graphic 78061 Version 5.0

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