Amount of starting RNA required | Sensitivity of test | Analytic requirements | Information obtained from the test | Number of targets | Relative cost | Advantages | Disadvantages | |
RT-PCR | Low | High | Low to moderate | Relative gene expression | Few to several hundred | Low |
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RNA in-situ hybridization | High | Low to moderate | Low | Presence and spatial localization of RNA of interest | Few | Low |
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Microarrays (oligonucleotide arrays) | Low to moderate | Moderate to high | Moderate to high | Relative gene expression | Thousands | Moderate |
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Custom microarrays | Moderate | Moderate to high | Moderate to high | Relative gene expression between two conditions | Several hundred | Moderate |
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Transcriptome sequencing | Low to moderate | High | Very high | Digital counts of each RNA molecule (as measure of gene expression) with excellent dynamic range | Unlimited | High |
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Northern blot | High | Low | Low | Presence and size of RNA of interest | Few | Low |
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Ribonuclease protection assay | High | Moderate | Low | Presence and size of RNA of interest | Few | Low |
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