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Instructions for optimal collection and handling of blood samples for measurement of tryptase following suspected anaphylaxis

Instructions for optimal collection and handling of blood samples for measurement of tryptase following suspected anaphylaxis
Tryptase (serum or plasma)
When to collect the sample:
After an episode of possible anaphylaxis, blood should be collected between 15 minutes and 3 hours after onset of signs or symptoms and optimally between 30 minutes and 2 hours after onset. Samples collected <15 minutes or >3 hours after clinical onset are less likely to be informative due to diminished sensitivity. However, a tryptase level associated with profound hypotension due to mast cell activation may be elevated for 3 to 7 hours (1 to 3 half-lives after a peak at 1 hour). For baseline levels, blood can be used that was collected prior to the event (eg, retrieved from a sample of serum or plasma refrigerated or frozen that was collected for a different lab test). Alternatively, a sample can be collected at least 24 hours after all signs and symptoms have resolved.
How to collect the sample:
Blood can be drawn using standard technique. Collect blood for serum (red top tube) or plasma (tube with heparin, citrate, or EDTA). A minimum of 1 mL is recommended.
For postmortem samples, collect blood from the femoral artery or vein, not the heart.
How to process the sample:
Serum of plasma should be placed on ice and frozen as soon as possible. Samples should be shipped frozen by overnight courier if the assay cannot be performed on site. If possible, acute and baseline samples should be analyzed in the same assay run to eliminate between-assay variation, although if paired samples are not available, more distant past or future samples can be used for the baseline level.
EDTA: ethylenediaminetetraacetic acid.
Graphic 72041 Version 27.0

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