DHR assay for CGD diagnosis. Unstimulated (left) and PMA-stimulated (right) neutrophils are shown. Y-axis is number of events; X-axis is fluorescence intensity shown on a log scale.
- In the normal (top panels), there is a rightward shift seen with stimulation.
- In the X-linked CGD carrier state (second row), there are two populations seen: one is normally fluorescent, while the other is essentially the same as the unstimulated population.
- In the patient with X-CGD (third row) there is no shift in fluorescence with stimulation.
- In the patient with the autosomal-recessive p47phox deficiency (fourth row), the rightward shift seen with stimulation is abnormally broad and not very bright.
Tracings courtesy of Dr. Douglas B Kuhns, SAIC Frederick.
