Version June 2024
| Examination of CSF |
| Cell count with differential |
| Protein |
| Glucose |
| Special stains (for specific pathogens, eosinophils) |
| Cytology |
| Cryptococcal antigen testing |
| Cultures (CSF, blood, other fluids where relevant) |
| Aerobic and anaerobic bacterial |
| Mycobacterial (including acid-fast stains) |
| Fungal |
| Serologies (blood and/or CSF) |
| Fungal serologic tests for Histoplasma, Coccidioides, Sporothrix, Cryptococcus (CF or immunodiffusion) |
| Toxoplasma serologies (IFA) |
| Antibody tests for HIV-1, HTLV I/II (ELISA, Western blot) |
| Cysticercosis serology (EITB) |
| Lyme serologies (ELISA, Western blot) |
| Syphilis serology (treponemal and nontreponemal tests) |
| Brucella serology (standard tube agglutination, CF, ELISA) |
| Tularemia serology |
| Imaging studies |
| Computed tomography (head and chest) |
| Magnetic resonance imaging of the head with gadolinium |
| Other |
| Tuberculin skin test or interferon-gamma release assay |
| Serum and CSF 1,3-beta-D-glucan assay |
| Meningeal or cortical biopsy (histopathology and culture) |
| Bone marrow aspiration and biopsy (with cultures) |
| Extractable nuclear antigens, antineutrophil cytoplasmic autoantibodies, and antibodies to cyclic citrullinated peptide |
| Erythrocyte sedimentation rate |
| Skin or lymph node biopsy, when applicable |
| Nucleic acid amplification testing of CSF for Mycobacterium tuberculosis |
| CSF PCR for enterovirus |
| CSF PCR for Tropheryma whipplei |
| CSF metagenomic next-generation sequencing or 16S rRNA sequencing |
| Ophthalmologic examination |
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