Comparison of cytogenetic, FISH, microarray, and next-generation sequencing techniques

Technique	Cytogenetic analysis	FISH analysis	Microarray analysis	Next-generation sequencing
Resolution	5 Mb	100 to 300 kb	Variable to few kb	Single nucleotide
Lower limit of detection	5%	~1 to 5%	15 to 20%	2 to 20%
Detects copy-neutral LOH	No	No	Yes	Maybe
Cell division required	Yes	No	No	No
Detects balanced translocations/inversions	Yes	Yes	No	Yes/No
Detects unbalanced aberrations, eg, deletions, unbalanced translocations	Yes	Yes	Yes	Yes/No
Detects multiple clones	Yes	Yes/No	No	Yes/No
Screen for novel lesions	Yes	No	Yes	Yes

FISH: fluorescence in situ hybridization; LOH: loss of heterozygosity.

Graphic 122979 Version 1.0

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